The REACH legislation encourages the development and use of Non-Testing Methods (NTM) for the assessment of hazards of substances. These alternative test methods include Quantitative Structure-Activity Relationship (QSAR) models, read-across, grouping and computer supported methodologies. Alternative methods are established to replace, reduce and refine animal testing, wherever possible. However, so far there is a deep gap of knowledge on which methods are available and can be used in practice.
Following the successful results of ANTARES, CALEIDOS aims to reduce this gap assessing NTM as an alternative approach for the REACH legislation.

Main Objectives

  • to provide practical information to the users of NTMs, based on exercises done on the registered chemicals;
  • to organise a statistically sound evaluation of the results in prediction of 23 QSAR methods, applying them to predict the properties of the chemicals registered to ECHA, and comparing the predicted values with the experimental ones in the registration data, for 6 properties: logP, bioconcentration factor, fish acute toxicity, mutagenicity (Ames test), carcinogenicity and reprotoxicity;
  • to evaluate if read across and grouping methods can improve the predictions, comparing the results from these NTMs with those obtained with QSAR for a set of selected chemicals;
  • to evaluate the reproducibility of the prediction using read across, organising an exercise with tens of experts;
  • to make freely available a web tool for the prediction of six chemical properties (logP, bioconcentration factor, fish acute toxicity, mutagenicity (Ames test), carcinogenicity, reprotoxicity);
  • to establish a network of tens of experts from regulatory bodies, industry and scientists, aiming to integrate the scientific and policy aspects;
  • to improve standardisation on the use of non-testing methods.



CALEIDOS is supported by

Position letter on read-across from FRANCOPA; FRANCOPA supports CALEIDOS

Position letter on read-across from FRANCOPA; FRANCOPA supports CALEIDOS

LIFE is the EU´s financial instrument supporting environmental and nature conservation projects throughout the EU, as well as in some candidate, acceding and neighbouring countries. Since 1992, LIFE has co-financed some 3708 projects, contributing approximately €2.8 billion to the protection of the environment.

  • July 27th 2015
    The Caleidos Final Results has been published
  • April 29th 2015
    Final Caleidos Workshop
  • May 27th 2015
    Workshop organized by SITOX: Approci in silico e strategie integrate nella valutazione del rischio